CyFlow™ CD8a Purified
Alternative Name: | Leu2, T8 |
Antibody: | Yes |
Antigen: | CD8a |
Application: | Flow cytometry, Functional assays, Immunohistochemistry, Immunoprecipitation, Western blot |
Clonality: | monoclonal |
Clone: | OX-8 |
Field of Interest: | Immunophenotyping, MHC |
Format/Fluorochrome: | Purified |
Isotype: | IgG1 |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Rat |
Product number: | CV104204 |
For Research Use Only
Concentration Unit | mg/mL |
Concentration | 1 |
Quantity | 0.1 mg |
Volume | 0.1 mL |
Immunogen | High Mw glycoproteins from rat thymocytes |
Background Information | CD8a (CD8 α) subunit of CD8 T cell coreceptor is expressed in CD8 α/β heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 α/α homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α/β but not α/α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. |
Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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