CyFlow™ CD11a PE
![CyFlow™ CD11a PE](https://la-en.sysmex-flowcytometry.com/media/image/0e/19/4b/Cyflow-bottle-2-with-RUO-label_600x600.png)
Alternative Name: | LFA-1 |
Antibody: | Yes |
Antigen: | CD11a |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | MEM-25 |
Emission Maximum: | 576 nm |
Excitation Maximum: | 496 nm, 565 nm |
Field of Interest: | Immunophenotyping |
Format/Fluorochrome: | PE |
Isotype: | IgG1 |
Laser: | Blue , Green, Yellow |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human |
Product number: | BK324379 |
For Research Use Only
Quantity | 100 tests |
Volume | 2.0 mL |
Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
Background Information | CD11a (LFA-1 α) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the αLβ2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
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