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CyFlow™ CD20 Purified

CyFlow™ CD20 Purified
Alternative Name: B1 , Bp35
Antibody: Yes
Antigen: CD20
Application: Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation
Clonality: monoclonal
Clone: 2H7
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: Purified
Isotype: IgG2b
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CR086124

For Research Use Only

HLDA Workshop HLDA IV—WS Code B201 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD20 Purified
HLDA WorkshopHLDA IV—WS Code B201
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman tonsillar B cells
Background InformationCD20 (B1, Bp35) is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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