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CyFlow™ CD28 Alexa Fluor™ 700

CyFlow™ CD28 Alexa Fluor™ 700
Alternative Name: Tp44
Antibody: Yes
Antigen: CD28
Application: Flow cytometry
Clonality: monoclonal
Clone: CD28.2
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 700
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CY207774

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen DC28.1.3.3 murine T cell hybridoma... more
CyFlow™ CD28 Alexa Fluor™ 700
Quantity100 tests
Volume0.4 mL
ImmunogenDC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Background InformationCD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Nunès J, Klasen S, Ragueneau M, Pavon C, Couez D, Mawas C, Bagnasco M, Olive D: CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol. 1993 Mar; 5(3):311‑5. < PMID: 8385476 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Nunes J, Klasen S, Franco MD, Lipcey C, Mawas C, Bagnasco M, Olive D: Signalling through CD28 T‑cell activation pathway involves an inosito phospholipid‑specific phospholipase C activity. Biochem J. 1993 Aug 1; 293 (3):835‑42. < PMID: 8394695 > | Galea-Lauri J, Darling D, Gan SU, Krivochtchapov L, Kuiper M, Gäken J, Souberbielle B, Farzaneh F: Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7‑mediated stimulation of NK cells. J Immunol. 1999 Jul 1; 163(1):62‑7. < PMID: 10384130 > | Tazi A, Moreau J, Bergeron A, Dominique S, Hance AJ, Soler P: Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol. 1999 Sep 15; 163(6):3511‑5. < PMID: 10477625 > | Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative‑feedback regulation of CD28 costimulation by a novel mitogen‑activated protein kinase phosphatase, MKP6. J Immunol. 2001 Jan 1; 166(1):197‑206. < PMID: 11123293 > | Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T‑cell activation negatively regulates NF‑kappa B signaling. Mol Cell Biol. 2004 May; 24(9):3860‑73. < PMID: 15082780 > | Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ: Immunization with hepatitis C virus‑like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004 Jul; 78(13):6995‑7003. < PMID: 15194776 >