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CyFlow™ CD29 Biotin

CyFlow™ CD29 Biotin
Alternative Name: Integrin β1
Antibody: Yes
Antigen: CD29
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-101A
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Dog, Human, Pig
Product number: BQ324745

For Research Use Only

HLDA Workshop HLDA VI—WS Code AS A048 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD29 Biotin
HLDA WorkshopHLDA VI—WS Code AS A048
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenRaji cells: human Burkitt's lymphoma cell line
Background InformationCD29 (β1 integrin subunit, GPIIa) forms non-covalently linked heterodimers with at least 6 different α chains (α1-α6, CD49a-f) determining the binding properties of β1 (VLA) integrins. These integrins mediate cell adhesion to collagen, fibronectin, laminin and other extracellular matrix (ECM) components. This interaction hinders cell death, whereas disruption of anchorage to ECM leads to apoptosis. Decreased expression of most β1 integrins correlates with acquiring multidrug resistance of tumor cells during selection in presence of antitumor drug. In platelets, translocation of intracellular pool of β1 integrins to the plasma membrane following thrombin stimulation. These integrins are also up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue, as well as in regulating tissue damage and disease symptoms related to inflammatory bowel disease. Through a β1 integrin-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1β.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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