CyFlow™ CD31 Alexa Fluor™ 488
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Alternative Name: | endoCAM, PECA1, PECAM-1 |
Antibody: | Yes |
Antigen: | CD31 |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | MEM-05 |
Emission Maximum: | 519 nm |
Excitation Maximum: | 495 nm |
Field of Interest: | Immunophenotyping |
Format/Fluorochrome: | Alexa Fluor™ 488 |
Isotype: | IgG1 |
Laser: | Blue |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human |
Product number: | AL676342 |
For Research Use Only
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
Background Information | CD31 (PECAM-1; platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15; 166(4):2364‑71. < PMID: 11160294 > | Newman DK, Hamilton C, Newman PJ: Inhibition of antigen‑receptor signaling by Platelet Endothelial Cell Adhesion Molecule‑1 (CD31 requires functional ITIMs, SHP‑2, and p56(lck). Blood. 2001 Apr 15; 97(8):2351‑7. < PMID: 11290597 > | Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule‑1 (PECAM‑1/CD31 acts as a regulator of B‑cell development, B‑cell antigen receptor (BCR)‑mediated activation, and autoimmune disease. Blood. 2002 Jul 1; 100(1):184‑9. < PMID: 12759520 > | Jackson DE: The unfolding tale of PECAM‑1. FEBS Lett. 2003 Apr 10; 540(1‑3):41821. < PMID: 12681475 > | Wee JL, Jackson DE: The Ig‑ITIM superfamily member PECAM‑1 regulates the "outside‑in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1; 106(12):3816‑23. < PMID: 16081692 > | Woodfin A, Voisin MB, Nourshargh S: PECAM‑1: a multi‑functional molecule in inflammation and vascular biolog. Arterioscler Thromb Vasc Biol. 2007 Dec; 27(12):2514‑23. < PMID: 17872453 > | Wong MX, Hayball JD, Jackson DE: PECAM‑1‑regulated signalling thresholds control tolerance in anergic transgenic B‑cells. Mol Immunol. 2008 Mar; 45(6):1767‑81. < PMID: 17977600 > | Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti‑CD31 antibodies. PLoS One. 2012; 7(12):e52815. < PMID: 23285192 >