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CyFlow™ CD33 APC-Cy7

CyFlow™ CD33 APC-Cy7
Alternative Name: p67 , SIGLECT-3
Antibody: Yes
Antigen: CD33
Application: Flow cytometry
Clonality: monoclonal
Clone: WM53
Emission Maximum: 780 nm
Excitation Maximum: 650 nm, 750 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC-Cy7
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BE790587

For Research Use Only

HLDA Workshop HLDA IV—WS Code M-505 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD33 APC-Cy7
HLDA WorkshopHLDA IV—WS Code M-505
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Acute Myelogenous Leukemia (AML) cells
Background InformationCD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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