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CyFlow™ CD34 PerCP

CyFlow™ CD34 PerCP
Antibody: Yes
Antigen: CD34
Application: Flow cytometry
Clonality: monoclonal
Clone: 581
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PerCP
Isotype: IgG1
Laser: Blue , Green
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CU690763

For Research Use Only

HLDA Workshop HLDA V—WS Code MA27 Quantity 100 tests Volume 1.0 mL Immunogen Not... more
CyFlow™ CD34 PerCP
HLDA WorkshopHLDA V—WS Code MA27
Quantity100 tests
Volume1.0 mL
ImmunogenNot available
Background InformationCD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15; 93(10):3379‑90. < PMID: 10233890 > | Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(‑)CD34(‑) cells in a long‑term in vitro system. Exp Hematol. 2000 Jun; 28(6):690‑9. < PMID: 10880755 > | Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15; 97(12):3768‑75. < PMID: 11389015 > | Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34‑ hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep; 28(6):587‑95. < PMID: 11607772 > | Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, andnormal bone marrow display different apoptosis and drug resistance‑associated phenotypes. Clin Cancer Res. 2004 Nov 15; 10(22):7599‑606. < PMID: 15569991 > | Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).. J Invest Dermatol. 2005 Jun; 124(6):1215‑24. < PMID: 15955097 > | Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c‑Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16; 4:9. < PMID: 16480521 > | Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose‑dependent contribution of CD34‑positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery aftr myocardial infarction. Circulation. 2006 Mar 14; 113(10):1311‑25. < PMID: 16534028 > | Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug; 94(8):1160‑3. < PMID: 19644143 > | Sanz E, Muñoz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10‑CD19+ pre/pro‑B‑cell and CD19‑ common lymphoid progenitor stages in two pro‑B‑cell development pathways. Proc Natl Acad Sci USA. 2010 Mar 30; 107(13):5925‑30. < PMID: 20231472 >