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CyFlow™ CD44 Purified

CyFlow™ CD44 Purified
Alternative Name: H-CAM , Pgp-1
Antibody: Yes
Antigen: CD44
Application: Flow cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: IM7
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgG2b
Regulatory Status: RUO
Source Species: Rat
Target Species: Cat, Dog, Horse, Human, Mouse
Product number: CK241123

For Research Use Only

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD44 Purified
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenDexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1
Background InformationCD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumor growth and metastasis, antiapoptosis and directional motility of cancer cells.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell‑surface glycoprotein. Immunogenetics. 1982 Mar; 15(3):313‑20. < PMID: 7068175 > | Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium‑neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar; 280(3):C423‑32. < PMID: 11171560 > | Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC‑regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun; 4(6):399‑407. < PMID: 12032545 > | McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation‑induced cell death: CD44‑deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep; 14(9):1015‑26. < PMID: 12202399 > | Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood‑retinal barrier. J Leukoc Biol. 2002 Dec; 72(6):1133‑41. < PMID: 12488494 > | Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen‑induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May; 111(10):1563‑70. < PMID: 12750406 > | van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6; 109(13):1647‑5. < PMID: 15023889 > | Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1; 177(1):268‑79. < PMID: 16785522 > | Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell‑T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul; 84(1):134‑42. < PMID: 18388297 >