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CyFlow™ CD45R APC

CyFlow™ CD45R APC
Antibody: Yes
Antigen: CD45R
Application: Flow cytometry
Clonality: monoclonal
Clone: RA3-6B2
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: APC
Isotype: IgG2a
Laser: Red
Regulatory Status: RUO
Source Species: Rat
Target Species: Cat, Human, Mouse
Product number: BJ993887

For Research Use Only

Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD45R APC
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.2 mL
ImmunogenAbelson murine leukemia virus-induced pre-B tumor cells
Background InformationCD45R is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Watanabe Y, Akaike T: Activation signal induces the expression of B cell‑specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May; 39(5):419‑25. < PMID: 7514808 > | Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2‑deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul; 9(1):135‑41. < PMID: 9697843 > | Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven‑color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May; 48(5):653‑62. < PMID: 10769049 > | Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220‑kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1; 172(7):4298‑307. < PMID: 15034044 > | Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B‑1 B cell‑specified progenitor. Nat Immunol. 2006 Mar; 7(3):293‑301. < PMID: 16429139 > | Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr; 69(4):249‑59. < PMID: 16538653 > | Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi‑infected joint tissue. J Immunol. 2012 Sep 1; 189(5):2488‑501. < PMID: 22851707 >