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CyFlow™ CD47 Purified

CyFlow™ CD47 Purified
Alternative Name: IAP
Antibody: Yes
Antigen: CD47
Application: Flow cytometry, Immunohistochemistry (frozen sections), Western blot
Clonality: monoclonal
Clone: MEM-122
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates, Pig
Product number: AV372758

For Research Use Only

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD47 Purified
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenCOS-7 African green monkey kidney cells
Background InformationCD47 (IAP; integrin-associated protein) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (SIRP α; signal regulatory protein α), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Barazi HO, Li Z, Cashel JA, Krutzsch HC, Annis DS, Mosher DF, Roberts DD: Regulation of integrin function by CD47 ligands: Differential effects on alphavbeta 3 and alpha 4beta1 integrin‑mediated adhesion. J Biol Chem. 2002 Nov 8; 277(45):42859‑66. < PMID: 12218055 > | Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD‑1 (SIRP‑1alpha) signaling pathway that inhibits LPS‑induced TNFalpha production by monocytes. Blood. 2003 Oct 1; 102(7):2532‑40. < PMID: 12805067 > | Murata T, Ohnishi H, Okazawa H, Murata Y, Kusakari S, Hayashi Y, Miyashita M, Itoh H, Oldenborg PA, Furuya N, Matozaki T: CD47 promotes neuronal development through Src‑ and FRG/Vav2‑mediated activation of Rac and Cdc42. J Neurosci. 2006 Nov 29; 26(48):12397‑407. < PMID: 17135401 > | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci USA. 2007 Mar 20; 104(12):5062‑6. < PMID: 17360380 > | Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD: Blockade of thrombospondin‑1‑CD47 interactions prevents necrosis of full thickness skin grafts. Ann Surg. 2008 Jan; 247(1):180‑90. < PMID: 18156939 > | Orazizadeh M, Lee HS, Groenendijk BC, Millward-Sadler J, Wright MO, Lindberg FP, Salter DM: CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model. Arthritis Res Ther. 2008 Jan 10; 10(1):R4. < PMID: 18186923 >