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CyFlow™ CD50 Alexa Fluor™ 488

CyFlow™ CD50 Alexa Fluor™ 488
Alternative Name: ICAM-3
Antibody: Yes
Antigen: CD50
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-171
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AD551837

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen Human granulocytes Background... more
CyFlow™ CD50 Alexa Fluor™ 488
Quantity100 tests
Volume0.4 mL
ImmunogenHuman granulocytes
Background InformationCD50 ( ICAM-3; intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (DC-SIGN; dendritic cell-specific ICAM-3-grabbing nonintegrin), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F: ICAM‑3 interacts with LFA‑1 and regulates the LFA‑1/ICAM‑1 cell adhesion pathway. J Cell Biol. 1993 Nov; 123(4):1007‑16. < PMID: 7901223 > | Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM‑3, the third LFA‑1 counterreceptor, is a co‑stimulatory molecule for both resting and activated T lymphocytes. Eur J Immunol. 1993 Nov; 23(11):2799‑806. < PMID: 8223855 > | Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM‑3 and LFA‑1‑mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J Cell Biol. 1994 Sep; 126(5):1277‑86. < PMID: 7520448 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8; 277(10):7955‑61. < PMID: 11773067 > | Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J Immunol Methods. 2007 Jan 30; 319(1‑2):21‑33. < PMID: 17098248 > | Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC‑SIGN binds ICAM‑3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007 Mar; 17(3):324‑33. < PMID: 17145745 > | Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J Biomed Biotechnol. 2010; 2010:841451. < PMID: 20339516 >