CyFlow™ CD53 Pacific Blue™
| Alternative Name: | TSPAN2 |
| Antibody: | Yes |
| Antigen: | CD53 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-53 |
| Emission Maximum: | 455 nm |
| Excitation Maximum: | 400 to 410 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Pacific Blue™ |
| Isotype: | IgG1 |
| Laser: | Violet |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AT117014 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Leukocytes of a patient suffering from LGL-type leukemia |
| Background Information | CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur J Immunol. 1986 Dec; 16(12):1583‑9. < PMID: 3493149 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Angelisová P, Vlcek C, Stefanova I, Lipoldova M, Horejsi V: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX‑44 antigens.. Immunogenetics. 1990; 32(4):281‑5. < PMID: 1700763 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15; 151(2):707‑16. < PMID: 8335905 > | Olweus J, Lund-Johansen F, Horejsi V: CD53, a protein with four membrane‑spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol. 1993 Jul 15; 151(2):707‑16. < PMID: 8335905 > | Rasmussen AM, Blomhoff HK, Stokke T, Horejsi V, Smeland EB: Cross‑linking of CD53 promotes activation of resting human B lymphocytes. J Immunol. 1994 Dec 1; 153(11):4997‑5007. < PMID: 7963560 > | Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S: Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol. 1996 Oct 1; 157(7):2939‑46. < PMID: 8816400 > | Mollinedo F, Martín-Martín B, Gajate C, Lazo PA: Physiological activation of human neutrophils down‑regulates CD53 cell surface antigen. J Leukoc Biol. 1998 Jun; 63(6):699‑706. < PMID: 9620662 > | Puls KL, Hogquist KA, Reilly N, Wright MD: CD53, a thymocyte selection marker whose induction requires a lower affinity TCR‑MHC interaction than CD69, but is up‑regulated with slower kinetics. Int Immunol. 2002 Mar; 14(3):249‑58. < PMID: 11867561 > | Yunta M, Rodríguez-Barbero A, Arévalo MA, López-Novoa JM, Lazo PA: Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primarycultures of mesangial cells. Kidney Int. 2003 Feb; 63(2):534‑42. < PMID: 12631118 > | Yunta M, Lazo PA: Apoptosis protection and survival signal by the CD53 tetraspanin antigen. Oncogene. 2003 Feb 27; 22(8):1219‑24. < PMID: 12606948 > | Kim TR, Yoon JH, Kim YC, Yook YH, Kim IG, Kim YS, Lee H, Paik SG: LPS‑induced CD53 expression: a protection mechanism against oxidative and radiation stress. Mol Cells. 2004 Feb 29; 17(1):125‑31. < PMID: 15055538 >