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CyFlow™ CD54 Alexa Fluor™ 700

CyFlow™ CD54 Alexa Fluor™ 700
Alternative Name: ICAM-1
Antibody: Yes
Antigen: CD54
Application: Flow cytometry
Clonality: monoclonal
Clone: 1H4
Emission Maximum: 723 nm
Excitation Maximum: 702 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 700
Isotype: IgG2b
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AE912016

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Raji cells and spleen cells fused with NS1... more
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CyFlow™ CD54 Alexa Fluor™ 700
Quantity100 tests
Volume0.4 mL
ImmunogenRaji cells and spleen cells fused with NS1 cells
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Boyd AW, Wawryk SO, Burns GF, Fecondo JV: Intercellular adhesion molecule 1 (ICAM‑1) has a central role in cell‑cell contact‑mediated immune mechanisms. Proc Natl Acad Sci USA. 1988 May; 85(9):3095‑9. < PMID: 3362863 > | Boyd AW, Dunn SM, Fecondo JV, Culvenor JG, Duhrsen U, Burns GF, Wawryk SO: Regulation of expression of a human intercellular adhesion molecule (ICAM‑1) during lymphohematopoietic differentiation. Blood. 1989 May 15; 73(7):1896‑903. < PMID: 2469503 > | Springer TA: Adhesion receptors of the immune system. Nature. 1990 Aug 2; 346(6283):425‑34. < PMID: 1974032 > | Ockenhouse CF, Betageri R, Springer TA, Staunton DE: Plasmodium falciparum‑infected erythrocytes bind ICAM‑1 at a site distinct from LFA‑1, Mac‑1, and human rhinovirus. Cell. 1992 Jan 10; 68(1):63‑9. < PMID: 1346257 > | Williams DT, Chaudhry Y, Goodfellow IG, Lea S, Evans DJ: Interactions of decay‑accelerating factor (DAF) with haemagglutinating human enteroviruses: utilizing variation in primate DAF to map virus binding sites. J Gen Virol. 2004 Mar; 85(3):731‑8. < PMID: 14993659 >

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