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CyFlow™ CD54 PE

CyFlow™ CD54 PE
Alternative Name: ICAM-1
Antibody: Yes
Antigen: CD54
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-111
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG2a
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cow, Human, Rat
Product number: AD327626

For Research Use Only

HLDA Workshop HLDA VI—WS Code AS A049 Quantity 100 tests Volume 2.0 mL Immunogen... more
CyFlow™ CD54 PE
HLDA WorkshopHLDA VI—WS Code AS A049
Quantity100 tests
Volume2.0 mL
ImmunogenRaji cells: human Burkitt's lymphoma cell line
Background InformationCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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