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CyFlow™ CD55 Alexa Fluor™ 488

CyFlow™ CD55 Alexa Fluor™ 488
Alternative Name: DAF
Antibody: Yes
Antigen: CD55
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-118
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgM
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CR455422

For Research Use Only

HLDA Workshop HLDA V—WS Code AS S016 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD55 Alexa Fluor™ 488
HLDA WorkshopHLDA V—WS Code AS S016
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD55 (DAF; decay-accelerating factor) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumors, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol‑anchored proteins and Lyn kinase. Blood. 1999 Feb 15; 93(4):1437‑9. < PMID: 10075459 > | Miwa T, Maldonado MA, Zhou L, Sun X, Luo HY, Cai D, Werth VP, Madaio MP, Eisenberg RA, Song WC: Deletion of decay‑accelerating factor (CD55 exacerbates autoimmune disease development in MRL/lpr mice. Am J Pathol. 2002 Sep; 161(3):1077‑86. < PMID: 12213736 > | Mikesch JH, Buerger H, Simon R, Brandt B: Decay‑accelerating factor (CD55): a versatile acting molecule in human malignancies. Biochim Biophys Acta. 2006 Aug; 1766(1):42‑52. < PMID: 16784816 > | Miwa T, Maldonado MA, Zhou L, Yamada K, Gilkeson GS, Eisenberg RA, Song WC: Decay‑accelerating factor ameliorates systemic autoimmune disease in MRL/lpr mice via both complement‑dependent and ‑independent mechanisms. Am J Pathol. 2007 Apr; 170(4):1258‑66. < PMID: 17392165 > | Abbott RJ, Spendlove I, Roversi P, Fitzgibbon H, Knott V, Teriete P, McDonnell JM, Handford PA, Lea SM: Structural and functional characterization of a novel T cell receptor co‑regulatory protein complex, CD97‑CD55. J Biol Chem. 2007 Jul 27; 282(30):22023‑32. < PMID: 17449467 > | VanLandingham JW, Cekic M, Cutler S, Hoffman SW, Stein DG: Neurosteroids reduce inflammation after TBI through CD55 induction. Neurosci Lett. 2007 Sep 25; 425(2):94‑8. < PMID: 17826908 >