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CyFlow™ CD58 Biotin

CyFlow™ CD58 Biotin
Alternative Name: LFA-3
Antibody: Yes
Antigen: CD58
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-63
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Pig
Product number: CE076390

For Research Use Only

HLDA Workshop HLDA VI—WS Code AS A047 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD58 Biotin
HLDA WorkshopHLDA VI—WS Code AS A047
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD58 (LFA-3) is an immunoglobulin family adhession molecule expressed by both hematopoietic and non-hematopoietic cells (often on antigen presenting cells) and serving as ligand of CD2. This interaction is important for T cell-mediated immunity. CD58 is expressed in transmembrane form and in GPI-anchored form; the later is constitutively associated with protein kinases whereas the transmembrane form activates kinase activity upon triggering. CD58 is a powerful tool for detection of minimal residual disease in acute lymphocytic leukemia, and for evaluation of liver damage related with hepatitis B.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Bayas MV, Schulten K, Leckband D: Forced detachment of the CD2‑CD58 complex. Biophys J. 2003 Apr; 84(4):2223‑33. < PMID: 12668431 > | Veltroni M, De Zen L, Sanzari MC, Maglia O, Dworzak MN, Ratei R, Biondi A, Basso G, Gaipa G: I‑BFM‑ALL‑FCM‑MRD‑Study Group: Expression of CD58 in normal, regenerating and leukemic bone marrow B cells: implications for the detection of minimal residual disease in acute lymphocytic leukemia. Haematologica. 2003 Nov; 88(11):1245‑52. < PMID: 14607753 > | Ariel O, Kukulansky T, Raz N, Hollander N: Distinct membrane localization and kinase association of the two isoforms of CD58. Cell Signal. 2004 Jun; 16(6):667‑73. < PMID: 15093607 > | Sheng L, Li J, Qi BT, Ji YQ, Meng ZJ, Xie M: Investigation on correlation between expression of CD58 molecule and severity of hepatitis B. World J Gastroenterol. 2006 Jul 14; 12(26):4237‑40. < PMID: 16830383 >