Article successfully added.

CyFlow™ CD59 Pacific Blue™

CyFlow™ CD59 Pacific Blue™
Alternative Name: Protectin H19
Antibody: Yes
Antigen: CD59
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-43
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Pacific Blue™
Isotype: IgG2a
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CT928190

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen Thymocytes and T lymphocytes Background... more
CyFlow™ CD59 Pacific Blue™
Quantity100 tests
Volume0.4 mL
ImmunogenThymocytes and T lymphocytes
Background InformationCD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia Biol (Praha). 1986; 32(1):12‑25. < PMID: 2422063 > | Forsberg UH, Bazil V, Stefanova I, Schroder J: Gene for human CD59 (likely Ly‑6 homologue) is located on the short arm of chromosome 11. Immunogenetics. 1989; 30(3):188‑93. < PMID: 2476389 > | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM‑43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 Feb; 26(2):153‑61. < PMID: 2918859 > | Stefanova I, Horejsi V, Ansotegui IJ, Knapp W, Stockinger H: GPI‑anchored cell‑surface molecules complexed to protein tyrosine kinases. Science. 1991 Nov 15; 254(5034):1016‑9. < PMID: 1719635 > | Cinek T, Horejsi V: The nature of large noncovalent complexes containing glycosyl‑phosphatidylinositol‑anchored membrane glycoproteins and protein tyrosine kinases.. J Immunol. 1992 Oct 1; 149(7):2262‑70. < PMID: 1382093 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Bodian DL, Davis SJ, Morgan BP, Rushmere NK: Mutational analysis of the active site and antibody epitopes of the complement‑inhibitory glycoprotein, CD59. J Exp Med. 1997 Feb 3; 185(3):507‑16. < PMID: 9053451 > | Cebecauer M, Cerny J, Horejsi V: Incorporation of leucocyte GPI‑anchored proteins and protein tyrosine kinases into lipid‑rich membrane domains of COS‑7 cells. Biochem Biophys Res Commun. 1998 Feb 24; 243(3):706‑10. < PMID: 9500981 > | Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar; 64(3):315‑26. < PMID: 12590976 > | Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly{09} lymphocyte cell surface receptor interacts homophilically through its N‑terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1; 174(11):7033‑42. < PMID: 15905546 > | Omidvar N, Wang EC, Brennan P, Longhi MP, Smith RA, Morgan BP: Expression of glycosylphosphatidylinositol‑anchored CD59 on target cells enhances human NK cell‑mediated cytotoxicity. J Immunol. 2006 Mar 1; 176(5):2915‑23. < PMID: 16493049 > | Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self‑MHC‑mediated Src family kinase activation. J Immunol. 2007 Feb 15; 178(4):2262‑71. < PMID: 17277131 > | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci USA. 2007 Mar 20; 104(12):5062‑6. < PMID: 17360380 > | Awasthi S, Wolf R, White G: Ontogeny and phagocytic function of baboon lung dendritic cells. Immunol Cell Biol. 2009 Jul; 87(5):419‑27. < PMID: 19223853 > | Sutherland DR, Keeney M, Illingworth A: Practical guidelines for the high‑sensitivity detection and monitoring of paroxysmal nocturnal hemoglobinuria (PNH) clones by flow cytometry. Cytometry B Clin Cytom. 2012 Jul; 82(4):195‑208. < PMID: 22499484 >