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CyFlow™ CD62L PerCP-Cy5.5

CyFlow™ CD62L PerCP-Cy5.5
Alternative Name: L-selectin
Antibody: Yes
Antigen: CD62L
Application: Flow cytometry
Clonality: monoclonal
Clone: DREG56
Emission Maximum: 695 nm
Excitation Maximum: 482 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PerCP-Cy5.5
Isotype: IgG1
Laser: Blue , Green
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BR497251

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen PMA-activated human peripheral blood... more
CyFlow™ CD62L PerCP-Cy5.5
Quantity100 tests
Volume0.4 mL
ImmunogenPMA-activated human peripheral blood leukocytes
Background InformationCD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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