CyFlow™ CD62L FITC
| Alternative Name: | L-selectin |
| Antibody: | Yes |
| Antigen: | CD62L |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | LT-TD180 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG1 |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BH999989 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Peripheral blood leukocytes |
| Background Information | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L‑selectin function is required for beta 2‑integrin‑mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct; 263(4‑2):H1034‑44. < PMID: 1384360 > | von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC: L‑selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX‑dependent and ‑independent recognition pathways. Blood. 1993 Jul 1; 82(1):182‑91. < PMID: 7686786 > | Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L‑selectin (CD62L) cross‑linking signals neutrophil adhesive functions via the Mac‑1 (CD11b/CD18 beta 2‑integrin. J Immunol. 1995 Aug 1; 155(3):1502‑14. < PMID: 7543524 > | Ding Z, Issekutz TB, Downey GP, Waddell TK: L‑selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1; 101(11):4245‑52. < PMID: 12609846 > | Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P‑selectin. Proc Natl Acad Sci USA. 2004 Sep 14; 101(37):13519‑24. < PMID: 15353601 > | Simone R, Zicca A, Saverino D: The frequency of regulatory CD3+CD8+CD28‑ CD25+ T lymphocytes in human peripheral blood increases with age. J Leukoc Biol. 2008 Dec; 84(6):1454‑61. < PMID: 18780874 > | Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8; 113(2):358‑69. < PMID: 18922855 > | Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J Biomed Biotechnol. 2010; 2010:841451. < PMID: 20339516 >