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CyFlow™ CD62L APC

CyFlow™ CD62L APC
Alternative Name: L-selectin
Antibody: Yes
Antigen: CD62L
Application: Flow cytometry
Clonality: monoclonal
Clone: LT-TD180
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AN470381

For Research Use Only

Quantity 100 tests Volume 1.0 mL Immunogen Peripheral blood leukocytes Background... more
CyFlow™ CD62L APC
Quantity100 tests
Volume1.0 mL
ImmunogenPeripheral blood leukocytes
Background InformationCD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE: L‑selectin function is required for beta 2‑integrin‑mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct; 263(4‑2):H1034‑44. < PMID: 1384360 > | von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC: L‑selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX‑dependent and ‑independent recognition pathways. Blood. 1993 Jul 1; 82(1):182‑91. < PMID: 7686786 > | Simon SI, Burns AR, Taylor AD, Gopalan PK, Lynam EB, Sklar LA, Smith CW: L‑selectin (CD62L) cross‑linking signals neutrophil adhesive functions via the Mac‑1 (CD11b/CD18 beta 2‑integrin. J Immunol. 1995 Aug 1; 155(3):1502‑14. < PMID: 7543524 > | Ding Z, Issekutz TB, Downey GP, Waddell TK: L‑selectin stimulation enhances functional expression of surface CXCR4 in lymphocytes: implications for cellular activation during adhesion and migration. Blood. 2003 Jun 1; 101(11):4245‑52. < PMID: 12609846 > | Ramachandran V, Williams M, Yago T, Schmidtke DW, McEver RP: Dynamic alterations of membrane tethers stabilize leukocyte rolling on P‑selectin. Proc Natl Acad Sci USA. 2004 Sep 14; 101(37):13519‑24. < PMID: 15353601 > | Simone R, Zicca A, Saverino D: The frequency of regulatory CD3+CD8+CD28‑ CD25+ T lymphocytes in human peripheral blood increases with age. J Leukoc Biol. 2008 Dec; 84(6):1454‑61. < PMID: 18780874 > | Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8; 113(2):358‑69. < PMID: 18922855 > | Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J Biomed Biotechnol. 2010; 2010:841451. < PMID: 20339516 >