CyFlow™ CD62L FITC
| Alternative Name: | L-selectin |
| Antibody: | Yes |
| Antigen: | CD62L |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEL-14 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping, MHC |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG2a |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Rat |
| Target Species: | Mouse |
| Product number: | AX041427 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,5 |
| Quantity | 0.1 mg |
| Volume | 0.2 mL |
| Immunogen | C3H/eb mouse B cell lymphoma 38C-13 |
| Background Information | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). |
| Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 6·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Gallatin WM, Weissman IL, Butcher EC: A cell‑surface molecule involved in organ‑specific homing of lymphocytes. Nature. 1983 Jul 7; 304:30‑34. < PMID: 6866086 > | Brawand P, Cerottini JC, MacDonald HR: Hierarchal utilization of different T‑cell receptor Vbeta gene segments in the CD8(+)‑T‑cell response to an immunodominant Moloney leukemia virus‑encoded epitope in vivo. J Virol. 1999 Nov; 73(11):9161‑9. < PMID: 10516023 > | ten Bruggencate SJ, Hillyer LM, Woodward BD: The proportion of CD45RA(+)CD62L(+) (quiescent‑phenotype) T cells within the CD8(+) subset increases in advanced weight loss in the protein‑ or energy‑deficient weanling mouse. J Nutr. 2001 Dec; 131(12):3266‑9. < PMID: 11739878 > | Mannering SI, Cheers C: Interleukin‑2 and loss of immunity in experimental Mycobacterium avium infection. Infect Immun. 2002 Jan; 70(1):27‑35. < PMID: 11748160 > | Friedline RH, Wong CP, Steeber DA, Tedder TF, Tisch R: L‑selectin is not required for T cell‑mediated autoimmune diabetes. J Immunol. 2002 Mar 15; 168(6):2659‑66. < PMID: 11884430 > | Fisson S, Darrasse-Jèze G, Litvinova E, Septier F, Klatzmann D, Liblau R, Salomon BL: Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state. J Exp Med. 2003 Sep 1; 198(5):737‑46. < PMID: 12939344 > | Chen BJ, Cui X, Sempowski GD, Liu C, Chao NJ: Transfer of allogeneic CD62L‑ memory T cells without graft‑versus‑host disease. Blood. 2004 Feb 15; 103(4):1534‑41. < PMID: 14551132 > | Chin CS, Miller CH, Graham L, Parviz M, Zacur S, Patel B, Duong A, Bear HD: Bryostatin 1/ionomycin (B/I) ex vivo stimulation preferentially activates L‑selectinlow tumor‑sensitized lymphocytes. Int Immunol. 2004 Sep; 16(9):1283‑94. < PMID: 15262898 > | Pop SM, Wong CP, He Q, Wang Y, Wallet MA, Goudy KS, Tisch R: The type and frequency of immunoregulatory CD4+ T‑cells govern the efficacy of antigen‑specific immunotherapy in nonobese diabetic mice. Diabetes. 2007 May; 56(5):1395‑402. < PMID: 17317763 > | Richards H, Longhi MP, Wright K, Gallimore A, Ager A: CD62L (L‑selectin) down‑regulation does not affect memory T cell distribution but failure to shed compromises anti‑viral immunity. J Immunol. 2008 Jan 1; 180(1):198‑206. < PMID: 18097020 > | Xu H, Manivannan A, Crane I, Dawson R, Liversidge J: Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation. Blood. 2008 Aug 15; 112(4):1166‑74. < PMID: 18391078 >