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CyFlow™ CD62P Biotin

CyFlow™ CD62P Biotin
Alternative Name: P-selectin
Antibody: Yes
Antigen: CD62P
Application: Flow cytometry
Clonality: monoclonal
Clone: AK4
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CU508361

For Research Use Only

HLDA Workshop HLDA VI—WS Code P-44 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD62P Biotin
HLDA WorkshopHLDA VI—WS Code P-44
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman platelets
Background InformationCD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP‑140 (P‑selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1; 175(4):1147‑50. < PMID: 1372646 > | Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan; 12(1):79‑89. < PMID: 9438413 > | Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co‑receptor CXCR4 on their surface: determination of response to stromal‑derived factor‑1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb; 104(2):220‑9. < PMID: 10050701 > | Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar; 122(3):830‑6. < PMID: 15086572 >