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CyFlow™ CD62P Low Endotoxin

CyFlow™ CD62P Low Endotoxin
Alternative Name: P-selectin
Antibody: Yes
Antigen: CD62P
Application: Flow cytometry, Functional assays, Immunocytochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: AK4
Field of Interest: Immunophenotyping
Format/Fluorochrome: Low Endotoxin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BR187242

For Research Use Only

Description Documentation TDS & SDS
Description
HLDA Workshop HLDA VI—WS Code P-44 Concentration Unit mg/mL Concentration 1... more
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CyFlow™ CD62P Low Endotoxin
HLDA WorkshopHLDA VI—WS Code P-44
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman platelets
Background InformationCD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP‑140 (P‑selectin) as a circulating plasma protein. J Exp Med. 1992 Apr 1; 175(4):1147‑50. < PMID: 1372646 > | Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB J. 1998 Jan; 12(1):79‑89. < PMID: 9438413 > | Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co‑receptor CXCR4 on their surface: determination of response to stromal‑derived factor‑1 by megakaryocytes and platelets. Br J Haematol. 1999 Feb; 104(2):220‑9. < PMID: 10050701 > | Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol. 2004 Mar; 122(3):830‑6. < PMID: 15086572 >

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