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CyFlow™ CD64 Alexa Fluor™ 647

CyFlow™ CD64 Alexa Fluor™ 647
Alternative Name: FcγRI
Antibody: Yes
Antigen: CD64
Application: Flow cytometry
Clonality: monoclonal
Clone: 10.1
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: CC859263

For Research Use Only

HLDA Workshop HLDA III—WS Code M-250 Quantity 100 tests Volume 0.4 mL Immunogen... more
CyFlow™ CD64 Alexa Fluor™ 647
HLDA WorkshopHLDA III—WS Code M-250
Quantity100 tests
Volume0.4 mL
ImmunogenRheumatoid synovial fluid cells and fibronectin purified human monocytes
Background InformationCD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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