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CyFlow™ CD71 APC

CyFlow™ CD71 APC
Alternative Name: T9 , TFR, TRFR
Antibody: Yes
Antigen: CD71
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-75
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Endocytosis, Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AM057841

For Research Use Only

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Description
HLDA Workshop HLDA IV—WS Code A 45 HLDA V—WS Code T T-165 Quantity 100 tests Volume... more
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CyFlow™ CD71 APC
HLDA WorkshopHLDA IV—WS Code A 45 HLDA V—WS Code T T-165
Quantity100 tests
Volume1.0 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford University Press, Oxford. 1989; 1‑1820. < NLM ID: 8914679 > | Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 1991 Mar 22; 251(5000):1451‑5. < PMID: 2006419 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan; 109(2):183‑92. < PMID: 11805130 > | Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA‑G proteins in cancer: do they provide tumor cells with an escape mechanism?. Cancer Res. 2005 Nov 15; 65(22):10139‑44. < PMID: 16287995 > | Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21; 13(35):4725‑36. < PMID: 17729394 > | Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol‑specific antibodies remodel HIV‑1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb; 51(2):286‑96. < PMID: 19654424 >

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