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CyFlow™ CD73 FITC

CyFlow™ CD73 FITC
Alternative Name: E5NT, Ecto'-5-nucleotidase , eN, eNT, NT5, NTE
Antibody: Yes
Antigen: CD73
Application: Flow cytometry
Clonality: monoclonal
Clone: AD2
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AY261195

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen Not available Background Information... more
CyFlow™ CD73 FITC
Quantity100 tests
Volume0.4 mL
ImmunogenNot available
Background InformationCD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Borrione P, Peola S, Mariani S, Besostri B, Mallone R, Malavasi F, Pileri A, Massaia M: CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3‑kinase pathway and inducing CD73 expression. J Immunol. 1999 May 15; 162(10):6238‑46. < PMID: 10229870 > | Nemoto E, Tada H, Shimauchi H: Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down‑regulation of chemokine production. J Leukoc Biol. 2002 Sep; 72(3):538‑45. < PMID: 12223522 > | Hashikawa T, Takedachi M, Terakura M, Saho T, Yamada S, Thompson LF, Shimabukuro Y, Murakami S: Involvement of CD73 (ecto‑5'‑nucleotidase) in adenosine generation by human gingival fibroblasts. J Dent Res. 2003 Nov; 82(11):888‑92. < PMID: 14578500 > | Rodriguez MW, Paquet AC, Yang YH, Erle DJ: Differential gene expression by integrin beta 7+ and beta 7‑ memory T helper cells. BMC Immunol. 2004 Jul 5; 5:13. < PMID: 15236665 > | Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cordblood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19; 200(2):123‑35. < PMID: 15263023 > | Paquet-Fifield S, Schlüter H, Li A, Aitken T, Gangatirkar P, Blashki D, Koelmeyer R, Pouliot N, Palatsides M, Ellis S, Brouard N, Zannettino A, Saunders N, Thompson N, Li J, Kaur P: A role for pericytes as microenvironmental regulators of human skin tissue regeneration. J Clin Invest. 2009 Sep; 119(9):2795‑806. < PMID: 19652362 > | Mokry J, Soukup T, Micuda S, Karbanova J, Visek B, Brcakova E, Suchanek J, Bouchal J, Vokurkova D, Ivancakova R: Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells. J Biomed Biotechnol. 2010; 2010:673513. < PMID: 20976265 > | Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A: Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol. 2010 Feb; 202(2):193. < PMID: 20035913 >