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CyFlow™ CD81 APC-Cy7

CyFlow™ CD81 APC-Cy7
Alternative Name: TAPA-1
Antibody: Yes
Antigen: CD81
Application: Flow cytometry
Clonality: monoclonal
Clone: M38
Emission Maximum: 780 nm
Excitation Maximum: 650 nm, 750 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC-Cy7
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cat, Human, Rabbit
Product number: AN580880

For Research Use Only

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Description
HLDA Workshop HLDA V—WS Code B070 Quantity 100 tests Volume 0.4 mL Immunogen... more
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CyFlow™ CD81 APC-Cy7
HLDA WorkshopHLDA V—WS Code B070
Quantity100 tests
Volume0.4 mL
ImmunogenMOLT-4 (human T-ALL cell line)
Background InformationCD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T‑cell leukemia virus type 1 (HTLV‑1)‑induced syncytium formation: altered glycosylation of C33 antigen in HTLV‑1‑positive T cells. J Virol. 1992 Mar; 66(3):1394‑401. < PMID: 1738199 > | Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1; 151(11):6470‑81. < PMID: 8245480 > | Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1; 155(3):1229‑39. < PMID: 7636191 > | Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B‑lymphocytes. J Biol Chem. 1998 Aug 7; 273(32):20121‑7. < PMID: 9685355 > | Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8‑color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2014 Apr; 36(2):165‑71. < PMID: 24028768 >

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