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CyFlow™ CD86 Pacific Orange™

CyFlow™ CD86 Pacific Orange™
Alternative Name: B7-2, B70
Antibody: Yes
Antigen: CD86
Application: Flow cytometry
Clonality: monoclonal
Clone: BU63
Emission Maximum: 551 nm
Excitation Maximum: 400 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Pacific Orange™
Isotype: IgG1
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AA524992

For Research Use Only

Quantity 100 tests Volume 0.4 mL Immunogen B-lymphoblastoid cell line ARH 77... more
CyFlow™ CD86 Pacific Orange™
Quantity100 tests
Volume0.4 mL
ImmunogenB-lymphoblastoid cell line ARH 77
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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