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CyFlow™ CD86 FITC

CyFlow™ CD86 FITC
Alternative Name: B7-2, B70
Antibody: Yes
Antigen: CD86
Application: Flow cytometry
Clonality: monoclonal
Clone: GL-1
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: FITC
Isotype: IgG2a
Laser: Blue
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: CQ603647

For Research Use Only

Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
CyFlow™ CD86 FITC
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.2 mL
ImmunogenLPS-activated CBA/Cs mouse splenic B cells
Background InformationCD86 (B7-2) and CD80 (B7-1) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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