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CyFlow™ CD95 Purified

CyFlow™ CD95 Purified
Alternative Name: APO-1, Fas , TNFRSF6
Antibody: Yes
Antigen: CD95
Application: Functional assays
Clonality: monoclonal
Clone: EOS9.1
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CP421037

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD95 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenP815 cells transfected with human CD95
Background InformationCD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Desbarats J, Birge RB, Mimouni-Rongy M, Weinstein DE, Palerme JS, Newell MK: Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nat Cell Biol. 2003 Feb; 5(2):118‑25. < PMID: 12545171 > | Conejo-Garcia JR, Benencia F, Courreges MC, Gimotty PA, Khang E, Buckanovich RJ, Frauwirth KA, Zhang L, Katsaros D, Thompson CB, Levine B, Coukos G: Ovarian carcinoma expresses the NKG2D ligand Letal and promotes the survival and expansion of CD28‑ antitumor T cells. Cancer Res. 2004 Mar 15; 64(6):2175‑82. < PMID: 15026360 > | Matsuoka K, Kim HT, McDonough S, Bascug G, Warshauer B, Koreth J, Cutler C, Ho VT, Alyea EP, Antin JH, Soiffer RJ, Ritz J: Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation. J Clin Invest. 2010 May; 120(5):1479‑93. < PMID: 20389017 > | Kasper HU, Konze E, Kern M, Stippel DL: CD95 and TNFα‑induced apoptosis in liver metastases of colorectal carcinoma. In Vivo. 2010 Sep-Oct; 24(5):653‑7. < PMID: 20952729 >

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