CyFlow™ CD98 Biotin
| Alternative Name: | 4F2 , FRP-1 |
| Antibody: | Yes |
| Antigen: | CD98 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-108 |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BH445832 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Raji cells: human Burkitt's lymphoma cell line |
| Background Information | CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with β1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Cho JY, Skubitz KM, Katz DR, Chain BM: CD98‑dependent homotypic aggregation is associated with translocation of protein kinase Cdelta and activation of mitogen‑activated protein kinases. Exp Cell Res. 2003 May 15; 286(1):1‑11. < PMID: 12729789 > | Liu X, Charrier L, Gewirtz A, Sitaraman S, Merlin D: CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT‑2 in polarized intestinal epithelia. J Biol Chem. 2003 Jun 27; 278(26):23672‑7. < PMID: 12716892 > | Cai S, Bulus N, Fonseca-Siesser PM, Chen D, Hanks SK, Pozzi A, Zent R: CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci. 2005 Mar 1; 118(5):889‑99. < PMID: 15713750 > | Dalton P, Christian HC, Redman CW, Sargent IL, Boyd CA: Differential effect of cross‑linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line. Biochim Biophys Acta. 2007 Mar; 1768(3):401‑10. < PMID: 17258169 >