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CyFlow™ CD98 Biotin

CyFlow™ CD98 Biotin
Alternative Name: 4F2 , FRP-1
Antibody: Yes
Antigen: CD98
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-108
Field of Interest: Immunophenotyping
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BH445832

For Research Use Only

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD98 Biotin
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenRaji cells: human Burkitt's lymphoma cell line
Background InformationCD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with β1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Cho JY, Skubitz KM, Katz DR, Chain BM: CD98‑dependent homotypic aggregation is associated with translocation of protein kinase Cdelta and activation of mitogen‑activated protein kinases. Exp Cell Res. 2003 May 15; 286(1):1‑11. < PMID: 12729789 > | Liu X, Charrier L, Gewirtz A, Sitaraman S, Merlin D: CD98 and intracellular adhesion molecule I regulate the activity of amino acid transporter LAT‑2 in polarized intestinal epithelia. J Biol Chem. 2003 Jun 27; 278(26):23672‑7. < PMID: 12716892 > | Cai S, Bulus N, Fonseca-Siesser PM, Chen D, Hanks SK, Pozzi A, Zent R: CD98 modulates integrin beta1 function in polarized epithelial cells. J Cell Sci. 2005 Mar 1; 118(5):889‑99. < PMID: 15713750 > | Dalton P, Christian HC, Redman CW, Sargent IL, Boyd CA: Differential effect of cross‑linking the CD98 heavy chain on fusion and amino acid transport in the human placental trophoblast (BeWo) cell line. Biochim Biophys Acta. 2007 Mar; 1768(3):401‑10. < PMID: 17258169 >