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CyFlow™ CD99R FITC

CyFlow™ CD99R FITC
Alternative Name: E2
Antibody: Yes
Antigen: CD99R
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-131
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgM
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AV675304

For Research Use Only

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Description
Quantity 100 tests Volume 2.0 mL Immunogen Human Acute Lymphocytic Leukemia (ALL) T... more
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CyFlow™ CD99R FITC
Quantity100 tests
Volume2.0 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T‑cell adhesion processes, isthe MIC2 gene product. EMBO J. 1989 Nov; 8(11):3253‑9. < PMID: 2479542 > | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+thymocytes. J Immunol. 1995 Jan 1; 154(1):26‑32. < PMID: 7527813 > | Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo‑monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1; 85(9):2402‑13. < PMID: 7537112 > | Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15; 158(6):2543‑50. < PMID: 9058785 > | Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up‑regulates alpha4beta1‑dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct; 30(10):3061‑5. < PMID: 11069091 > | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8; 277(10):7955‑61. < PMID: 11773067 >

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