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CyFlow™ CD107b APC

CyFlow™ CD107b APC
Alternative Name: LAMP-2
Antibody: Yes
Antigen: CD107b
Application: Flow cytometry
Clonality: monoclonal
Clone: H4B4
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CT778572

For Research Use Only

Quantity 100 tests Volume 1.0 mL Immunogen Human peripheral blood mononuclear cells... more
CyFlow™ CD107b APC
Quantity100 tests
Volume1.0 mL
ImmunogenHuman peripheral blood mononuclear cells (PBMC)
Background InformationCD107b (LAMP-2; lysosome-associated membrane protein-2), together with CD107a (LAMP-1), is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.02% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

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