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CyFlow™ CD108 FITC

CyFlow™ CD108 FITC
Alternative Name: GPI-gp80, JMH, SEMA7A , Sema K1
Antibody: Yes
Antigen: CD108
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-150
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Blood group antigens, Immunophenotyping
Format/Fluorochrome: FITC
Isotype: IgM
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BU957025

For Research Use Only

Quantity 100 tests Volume 2.0 mL Immunogen Human Acute Lymphocytic Leukemia (ALL) T... more
CyFlow™ CD108 FITC
Quantity100 tests
Volume2.0 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD108 (SEMA7A; Semaphorin 7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors - plexin C1 and β1 integrins.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr; 46(4):334‑6. < PMID: 7684403 > | Mudad R, Rao N, Angelisova P, Horejsi V, Telen MJ: Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion. 1995 Jul; 35(7):566‑70. < PMID: 7631388 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Angelisová P, Drbal K, Cerny J, Hilgert I, Horejsi V: Characterization of the human leukocyte GPI‑anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999 Jun; 200(2):234‑45. < PMID: 10416131 > | Pasterkamp RJ, Peschon JJ, Spriggs MK, Kolodkin AL: Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature. 2003 Jul 24; 424(6947):398‑405. < PMID: 12879062 > | Delorme G, Saltel F, Bonnelye E, Jurdic P, Machuca-Gayet I: Expression and function of semaphorin 7A in bone cells. Biol Cell. 2005 Jul; 97(7):589‑97. < PMID: 15859945 > | Suzuki K, Okuno T, Yamamoto M, Pasterkamp RJ, Takegahara N, Takamatsu H, Kitao T, Takagi J, Rennert PD, Kolodkin AL, Kumanogoh A, Kikutani H: Semaphorin 7A initiates T‑cell‑mediated inflammatory responses through alpha1beta1 integrin. Nature. 2007 Apr 5; 446(7136):680‑4. < PMID: 17377534 > | Pasterkamp RJ, Kolk SM, Hellemons AJ, Kolodkin AL: Expression patterns of semaphorin7A and plexinC1 during rat neural development suggest roles in axon guidance and neuronal migration. BMC Dev Biol. 2007 Aug 29; 7:98. < PMID: 17727705 >