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CyFlow™ CD112 PE

CyFlow™ CD112 PE
Alternative Name: Hve B, HVEB, Nectin-2, PRR2
Antibody: Yes
Antigen: CD112
Application: Flow cytometry
Clonality: monoclonal
Clone: R2.525
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AL095410

For Research Use Only

Quantity 100 tests Volume 1.0 mL Immunogen Not available Background Information... more
CyFlow™ CD112 PE
Quantity100 tests
Volume1.0 mL
ImmunogenNot available
Background InformationCD112 (nectin-2) is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule‑1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1; 67(3):1317‑25. < PMID: 17283169 > | Enqvist M, Nilsonne G, Hammarfjord O, Wallin RP, Björkström NK, Björnstedt M, Hjerpe A, Ljunggren HG, Dobra K, Malmberg KJ, Carlsten M: Selenite induces posttranscriptional blockade of HLA‑E expression and sensitizes tumor cells to CD94/NKG2A‑positive NK cells.. J Immunol. 2011 Oct 1; 187(7):3546‑54. < PMID: 21890659 > | Nielsen N, Ødum N, Ursø B, Lanier LL, Spee P: Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA‑1 and TRAIL and dampened via CD94/NKG2A. PLoS One. 2012; 7(2):e31959. < PMID: 22384114 > | Hou S, Ge K, Zheng X, Wei H, Sun R, Tian Z: CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain. J Biol Chem. 2014 Mar 7; 289(10):6969‑77. < PMID: 24451371 >