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CyFlow™ CD129 PE

CyFlow™ CD129 PE
Alternative Name: IL-9Ra , IL9R
Antibody: Yes
Antigen: CD129
Application: Flow cytometry
Clonality: monoclonal
Clone: AH9R7
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CE042379

For Research Use Only

Quantity 100 tests Volume 1.0 mL Immunogen Human CD129-transfected cells... more
CyFlow™ CD129 PE
Quantity100 tests
Volume1.0 mL
ImmunogenHuman CD129-transfected cells
Background InformationCD129 serves as the high affinity α subunit of IL-9 receptor (IL-9R α). It associates with CD132, the common γ chain shared by receptors of many different cytokines. CD129 is expressed at low levels by T and B cells, blood cell progenitors, eosinophils, mast cells, epithelial cells, muscle cells and neurons. Its signaling (through JAK/STAT pathways) results in proliferative and anti-apoptotic response, which is critical e.g. for intrathymic T cell development and survival of various cell types. The gene for CD129 is located at the pseudoautosomal regions of X and Y chromosomes and it may be related with the development of asthma.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Grasso L, Huang M, Sullivan CD, Messler CJ, Kiser MB, Dragwa CR, Holroyd KJ, Renauld JC, Levitt RC, Nicolaides NC: Molecular analysis of human interleukin‑9 receptor transcripts in peripheral blood mononuclear cells: Identification of a splice variant encoding for a nonfunctional cellsurface receptor. J Biol Chem. 1998 Sep 11; 273(37):24016‑24. < PMID: 9727018 > | De Smedt M, Verhasselt B, Kerre T, Vanhecke D, Naessens E, Leclercq G, Renauld JC, Van Snick J, Plum J: Signals from the IL‑9 receptor are critical for the early stages of human intrathymic T cell development. J Immunol. 2000 Feb 15; 164(4):1761‑7. < PMID: 10657622 > | Pilette C, Ouadrhiri Y, Van Snick J, Renauld JC, Staquet P, Vaerman JP, Sibille Y: IL‑9 inhibits oxidative burst and TNF‑alpha release in lipopolysaccharide‑stimulated human monocytes through TGF‑beta. J Immunol. 2002 Apr 15; 168(8):4103‑11. < PMID: 11937570 > | Pilette C, Ouadrhiri Y, Van Snick J, Renauld JC, Staquet P, Vaerman JP, Sibille Y: Oxidative burst in lipopolysaccharide‑activated human alveolar macrophages is inhibited by interleukin‑9. Eur Respir J. 2002 Nov; 20(5):1198‑205. < PMID: 12449174 >