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CyFlow™ CD140a Biotin

CyFlow™ CD140a Biotin
Alternative Name: PDGFRA , PDGRF2
Antibody: Yes
Antigen: CD140a
Application: Flow cytometry
Clonality: monoclonal
Clone: 16A1
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AS196282

For Research Use Only

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ CD140a Biotin
Concentration Unitmg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenCD140a-transfected NIH 3T3 cells
Background InformationCD140a (PDGF-RA; platelet-derived growth factor receptor α) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b (PDGF-RB). Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep; 78(3):725‑35. < PMID: 16000396 > | Andrae J, Gallini R, Betsholtz C: Role of platelet‑derived growth factors in physiology and medicine. Genes Dev. 2008 May 15; 22(10):1276‑312. < PMID: 18483217 > | French WJ, Creemers EE, Tallquist MD: Platelet‑derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol. 2008 Sep; 28(18):5646‑57. < PMID: 18606782 > | Schmahl J, Rizzolo K, Soriano P: The PDGF signaling pathway controls multiple steroid‑producing lineages. Genes Dev. 2008 Dec 1; 22(23):3255‑67. < PMID: 19056881 > | Faraone D, Aguzzi MS, Toietta G, Facchiano AM, Facchiano F, Magenta A, Martelli F, Truffa S, Cesareo E, Ribatti D, Capogrossi MC, Facchiano A: Platelet‑derived growth factor‑receptor alpha strongly inhibits melanoma growth in vitro and in vivo. Neoplasia. 2009 Aug; 11(8):732‑42. < PMID: 19649203 >