CyFlow™ Bcl2 FITC
Antibody: | Yes |
Antigen: | Bcl2 |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | Bcl-2/100 |
Emission Maximum: | 518 nm |
Excitation Maximum: | 490 to 495 nm |
Field of Interest: | Apoptosis |
Format/Fluorochrome: | FITC |
Isotype: | IgG1 |
Laser: | Blue |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human |
Product number: | AY147314 |
For Research Use Only
Quantity | 100 tests |
Volume | 0.4 mL |
Immunogen | Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2 |
Background Information | Bcl2 (B cell CLL lymphoma 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms. |
Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl‑2‑independent pathway. J Neurosci. 1999 Jun 15; 19(12):4828‑38. < PMID: 10366617 > | Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb; 21(2):225‑31. < PMID: 12608434 > | Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl‑2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul; 13(7):1235‑7. < PMID: 16485035 > | Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct; 81(20):11426‑40. < PMID: 17670831 > | Joubert A, Marais S, Maritz C: Influence of 2‑methoxyestradiol on MCF‑7 cells: an improved differential interference contrasting technique and Bcl‑2 and Bax protein expression levels. Biocell. 2009 Apr; 33(1):67‑70. < PMID: 19499887 >