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CyFlow™ Notch 1 APC

CyFlow™ Notch 1 APC
Antibody: Yes
Antigen: Notch 1
Application: Flow cytometry
Clonality: monoclonal
Clone: mN1A
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Transcription
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Mouse
Product number: BB063499

For Research Use Only

Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
CyFlow™ Notch 1 APC
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenGST fusion protein containing cdc10-NCR region of mouse Notch 1
Background InformationNotch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing‑deficient allele of Notch1. Nature. 2000 Jun 22; 405(6789):966‑70. < PMID: 10879540 > | Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65‑NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N‑CoR. J Cell Sci. 2002 Mar 15; 115(6):1295‑303. < PMID: 11884528 > | Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21; 177(4):647‑57. < PMID: 17502421 > | Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch‑1. Mol Cell Biol. 2008 Jan; 28(1):165‑76. < PMID: 17967888 > | Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto‑1. J Cell Biol. 2009 Nov 2; 187(3):343‑53. < PMID: 19948478 > | Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR‑326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J Neurosci. 2009 Dec 2; 29(48):15161‑8. < PMID: 19955368 > | Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV‑1 Rev‑binding protein accelerates cellular uptake of iron to drive Notch‑induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1; 120(7):2537‑48. < PMID: 20516639 >