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CyFlow™ STRO1 PE

CyFlow™ STRO1 PE
Antibody: Yes
Antigen: STRO1
Application: Flow cytometry
Clonality: monoclonal
Clone: STRO-1
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgM
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BA518386

For Research Use Only

Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
CyFlow™ STRO1 PE
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenHuman CD34 positive bone marrow cells
Background InformationSTRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO‑1. Blood. 1991 Jul 1; 78(1):55‑62. < PMID: 2070060 > | Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO‑1 monoclonal antibody. J Bone Miner Res. 1999 Mar; 14(3):351‑61. < PMID: 10027900 > | Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001 Jun; 16(6):1120‑9. < PMID: 11393789 > | Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003 May 1; 116(9):1827‑35. < PMID: 12665563 > | Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro‑1‑ or Stro‑1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004 May 1; 103(9):3313‑9. < PMID: 14715641 > | Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005 Oct; 84(10):907‑12. < PMID: 16183789 > | Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO‑1 antigen in developing rat teeth by light microscopy and electron microscopy. J Electron Microsc (Tokyo). 2009 Dec; 58(6):363‑73. < PMID: 19525367 >