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CyFlow™ STRO1 Purified

CyFlow™ STRO1 Purified
Antibody: Yes
Antigen: STRO1
Application: Flow cytometry, Immunocytochemistry
Clonality: monoclonal
Clone: STRO-1
Field of Interest: Immunophenotyping
Format/Fluorochrome: Purified
Isotype: IgM
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BB174126

For Research Use Only

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ STRO1 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman CD34 positive bone marrow cells
Background InformationSTRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Storage BufferThe reagent is provided in Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO‑1. Blood. 1991 Jul 1; 78(1):55‑62. < PMID: 2070060 > | Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO‑1 monoclonal antibody. J Bone Miner Res. 1999 Mar; 14(3):351‑61. < PMID: 10027900 > | Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001 Jun; 16(6):1120‑9. < PMID: 11393789 > | Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003 May 1; 116(9):1827‑35. < PMID: 12665563 > | Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro‑1‑ or Stro‑1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004 May 1; 103(9):3313‑9. < PMID: 14715641 > | Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005 Oct; 84(10):907‑12. < PMID: 16183789 > | Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO‑1 antigen in developing rat teeth by light microscopy and electron microscopy. J Electron Microsc (Tokyo). 2009 Dec; 58(6):363‑73. < PMID: 19525367 >